DMEM/F-12 Cell Culture Media

DMEM/F-12 cell culture media is a serum-free formulation that combines Dulbecco's modified Eagle medium (DMEM) and Ham's F-12 nutrient mixture in a 1:1 ratio. It is designed to provide a suitable environment for the growth and maintenance of various cell types in laboratory settings. This medium is enriched with essential nutrients, including insulin, transferrin, and growth factors, supporting the optimal proliferation and survival of cells1. DMEM/F-12 cell culture media has been widely used for culturing primary mammalian cells as well as various normal and transformed cell lines. Its versatility and ability to support a broad range of cell types make it a valuable tool in cellular research and biotechnological applications.

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Corning™ Basal Cell Culture Liquid Media - DMEM and Ham's F-12, 50/50 Mix

Used for culture of epithelial, endothelial, and granulosa cell types under low serum conditions. Corning™ Basal Cell Culture Liquid Media - DMEM and Ham's F-12, 50/50 Mix has low levels of endotoxin, and absence of contamination.

Pricing & Availability
Specifications

Content And Storage	2° to 8°C
Product Type	DMEM and Ham's F-12, 50/50 Mix
Form	Liquid
Endotoxin Level	<0.25EU/mL
pH	7 to 7.4
Shelf Life	12 Months
Concentration	1 X
For Use With (Application)	To assess growth promotion and cytotoxicity characteristics
Sterility	Sterile

Cytiva HyClone™ DMEM/F12 1:1 Media

Used for culturing many different mammalian cells including MDCK, Glial cells, fibroblasts, and endothelial cells. HyClone™ DMEM/F12 1:1 Media supports cell culture growth and biomanufacturing processes and is manufactured using ISO 9001:2000-certified processes.

Pricing & Availability
Specifications

Content And Storage	2° to 8°C, away from light
Sterilization Method	0.1 μm Sterile-filtered
Form	Liquid
Shelf Life	12 Months
Certifications/Compliance	ISO 9001:2000
Sterility	Sterile-filtered

Gibco™ DMEM/F-12, HEPES

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Pricing & Availability
Specifications

With Additives	High Glucose,Glutamine,HEPES,Phenol Red,Sodium Pyruvate
Product Type	DMEM (Dulbecco's Modified Eagle Medium)/F-12
Form	Liquid
Classification	Animal Origin-free
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered

Gibco™ Advanced DMEM/F-12

Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	MRC-5, SP2, Vero, WI-38 and Jurkat
Content And Storage	Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture
Form	Liquid
Without Additives	No Glutamine,No HEPES
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Green Features	Sustainable packaging
Concentration	1 X
Sterility	Sterile-filtered
With Additives	High Glucose,Phenol Red,Sodium Pyruvate,Non Essential Amino Acids (NEAA)
Product Type	DMEM (Dulbecco's Modified Eagle Medium)/F-12
Classification	Animal Origin
Shelf Life	12 Months From Date of Manufacture
Product Line	Gibco™
Serum Level	Reduced Serum

Gibco™ DMEM/F-12, no phenol red

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Pricing & Availability

Gibco™ DMEM/F-12, GlutaMAX™ supplement

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Pricing & Availability
Specifications

With Additives	High Glucose,GlutaMAX,Phenol Red,Sodium Pyruvate
Product Type	DMEM (Dulbecco's Modified Eagle Medium)/F-12
Form	Liquid
Without Additives	No HEPES
Classification	Animal Origin-free
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered

Gibco™ DMEM/F-12

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
Content And Storage	Storage conditions: 2°C to 8°C (protect from light) Shipping conditions: Ambient Shelf life: 12 months from date of manufacture
Form	Liquid
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Green Features	Sustainable packaging
Concentration	1 X
Sterility	Sterile-filtered
Cell Type	Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
With Additives	High Glucose,Glutamine,Phenol Red,Sodium Pyruvate
Product Type	DMEM (Dulbecco's Modified Eagle Medium)/F-12
Classification	Animal Origin-free
Shelf Life	12 Months From Date of Manufacture
Product Line	Gibco™
Serum Level	Standard Serum Supplementation

Gibco™ DMEM/F-12, HEPES, no phenol red

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Pricing & Availability
Specifications

Cell Line	MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
Content And Storage	Storage conditions: 2°C to 8°C (protect from light) Shipping conditions: Ambient Shelf life: 12 months from date of manufacture
Form	Liquid
Without Additives	No Phenol Red
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Green Features	Sustainable packaging
Concentration	1X
Sterility	Sterile-filtered
With Additives	High Glucose,Glutamine,HEPES
Product Type	DMEM (Dulbecco's Modified Eagle Medium)/F-12
Classification	Animal Origin-free
Shelf Life	12 Months From Date of Manufacture
Product Line	Gibco™
Serum Level	Standard Serum Supplementation

Gibco™ BenchStable™ DMEM/F12

Gibco DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
Content And Storage	Store at room temperature. Protect from light.
Form	Liquid
Without Additives	No HEPES
Manufacturing Quality	cGMP-compliant under the ISO 9001: 2015 standard
Green Features	Energy efficient, Fewer resources used, Sustainable packaging
Concentration	1 X
Sterility	Sterile-filtered
With Additives	High Glucose,GlutaMAX,Phenol Red,Sodium Pyruvate
Product Type	DMEM (Dulbecco's Modified Eagle Medium)/F-12
Classification	Animal Origin-free
Shelf Life	12 Months From Date of Manufacture
Product Line	BenchStable™
Serum Level	Standard Serum Supplementation

Gibco™ CTS™ KnockOut™ DMEM/F-12

CTS KnockOut DMEM/F-12 CTS is a serum free, low osmolality medium without L-glutamine or HEPES buffer that is optimized for the growth of human embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC).

Pricing & Availability

MilliporeSigma™ DMEM/F-12 PLUS Basal Medium

Advanced DMEMF12 basal media used in low-serum or serum-free media formulations

Pricing & Availability

Gibco™ KnockOut™ DMEM/F-12

KnockOut™ DMEM/F-12

Pricing & Availability

Gibco™ DMEM/F-12, powder, HEPES

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Pricing & Availability

Gibco™ DMEM/F-12, no glutamine

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM/F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Pricing & Availability
Specifications

Form	Liquid
Without Additives	No Glutamine,No HEPES
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Shelf Life	12 Months

Gibco™ DMEM/F-12, powder

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Content And Storage	Storage conditions: 2-8° C Shipping conditions: Ambient Shelf life: 36 months from date of manufacture
With Additives	High Glucose,Glutamine,Phenol Red,Sodium Pyruvate
Form	Powder
Without Additives	No HEPES
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Product Line	Gibco™
Serum Level	Standard Serum Supplementation

DMEM/F-12 Cell Culture Media

DMEM/F-12 Cell Culture Media

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Corning™ Basal Cell Culture Liquid Media - DMEM and Ham's F-12, 50/50 Mix SureTRACESupports traceability with guaranteed access to certificates and proactive change notifications.Learn More

Gibco™ DMEM/F-12, HEPES Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ Advanced DMEM/F-12 Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ DMEM/F-12, no phenol red Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ DMEM/F-12, GlutaMAX™ supplement Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ DMEM/F-12 Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ DMEM/F-12, HEPES, no phenol red Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ BenchStable™ DMEM/F12 Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ CTS™ KnockOut™ DMEM/F-12 Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ KnockOut™ DMEM/F-12 Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ DMEM/F-12, no glutamine Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Corning™ Basal Cell Culture Liquid Media - DMEM and Ham's F-12, 50/50 Mix

Gibco™ DMEM/F-12, HEPES

Gibco™ Advanced DMEM/F-12

Gibco™ DMEM/F-12, no phenol red

Gibco™ DMEM/F-12, GlutaMAX™ supplement

Gibco™ DMEM/F-12

Gibco™ DMEM/F-12, HEPES, no phenol red

Gibco™ BenchStable™ DMEM/F12

Gibco™ CTS™ KnockOut™ DMEM/F-12

Gibco™ KnockOut™ DMEM/F-12

Gibco™ DMEM/F-12, no glutamine