Gibco™ DMEM/F-12

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Catalog Number	Mfr. No.	Quantity
11-320-033	Gibco™ 11320033	500 mL
11-320-082	Gibco™ 11320082	10 x 500 mL

Description

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM/F-12 is modified as follows:
With: L-glutamine, Phenol Red
Without: HEPES

The complete formulation is available.

Using DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage: Storage conditions: 2°C to 8°C (protect from light)
  Shipping conditions: Ambient
  Shelf life: 12 months from date of manufacture
• Cell Type: Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
• Concentration: 1 X
• Product Type: DMEM (Dulbecco's Modified Eagle Medium)/F-12
• Sterility: Sterile-filtered
• Green Features: Sustainable packaging
• Product Line: Gibco™

• Cell Line: MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
• Classification: Animal Origin-free
• Form: Liquid
• Serum Level: Standard Serum Supplementation
• With Additives: High Glucose, Glutamine, Phenol Red, Sodium Pyruvate
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Shipping Condition: Room Temperature

Safety and Handling

ShelfLife : 12 Months From Date of Manufacture

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.