Buffers and Standards
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Buffers and Standards
Hydrogen ion buffers comprising a mixture of a weak acid and its conjugate base or vice-versa and used to stabilize pH; also diluents, washing solutions, and standard-value solutions used for a broad array of scientific calibration purposes.
The buffers used to calibrate pH meters may be certified and/or traceable to the National Institute of Standards and Technology (NIST). These buffers may also be color-coded for easy identification:
- Red: pH 4.0
- Yellow: pH 7.0
- Blue: pH 10.0
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Filtered Search Results
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Buffer Solution, pH 7.00 (Certified), Fisher Chemical™
Potassium Phosphate Monobasic/Sodium Hydroxide
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Color | Colorless |
---|---|
Traceability to NIST | Traceable to NIST. |
Physical Form | Liquid |
Chemical Name or Material | Buffer Solution, pH 7.00 |
Grade | Certified |
Identification | Pass Test |
ChemAlert Storage Symbol | Gray |
Density | 1.003g/cm³ |
Vapor Pressure | 14mmHg at 20°C |
CAS | 7732-18-5 |
MDL Number | MFCD00134130 |
pH | 7.00 |
Synonym | Chlorine Titrimeter Reagent |
Melting Point | 0°C |
Buffer Solution, pH 10.00 (Certified), Fisher Chemical™
Potassium Carbonate/Potassium Tetraborate/Potassium Hydroxide/Disodium EDTA Dihydrate
![EDGE](/content/dam/fishersci/glyphs/edge.png)
CAS | 12228-88-5 |
---|---|
Health Hazard 3 | Emergency Overview May cause eye, skin, and respiratory tract irritation. Handle in accordance with good industrial hygiene and safety practice. Wear personal protective equipment. Wash off immediately with plenty of water for at least 15 minutes. Get medical attention immediately if symptoms occur. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Get medical attention immediately if symptoms occur. Do not induce vomiting. Obtain medical attention. Obtain medical attention. . NFPA Health:1 Flammability:0 Instability:0 |
MDL Number | MFCD00146206 |
Health Hazard 2 | CAUTION! |
Color | Colorless |
Traceability to NIST | Traceable to NIST. |
Physical Form | Liquid |
pH | 10 |
Chemical Name or Material | Buffer Solution, pH 10.00 |
Grade | Certified |
Identification | Pass Test |
ChemAlert Storage Symbol | Gray |
Buffer, Reference Standard, pH 4.00 ± 0.01 at 25°C (Color Coded Red), Ricca Chemical
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![EDGE](/content/dam/fishersci/glyphs/edge.png)
Name Note | pH 4.00 ± 0.01 at 25 deg C (Color Coded Red) |
---|---|
CAS | 7732-18-5 |
CAS Min % | 0.99 |
Chemical Name or Material | Buffer, Reference Standard |
CAS Max % | 1.03 |
Buffer, Reference Standard, pH 10.00 ± 0.01 at 25°C (Color Coded Blue), Ricca Chemical
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![BIOPHARMA](/content/dam/fishersci/glyphs/sure_trace.png)
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![EDGE](/content/dam/fishersci/glyphs/edge.png)
Boiling Point | 100°C |
---|---|
Name Note | pH 10.00 ± 0.01 at 25 deg C (Color Coded Blue) |
CAS | 7732-18-5 |
Color | Blue |
Solubility Information | Miscible |
Physical Form | Liquid |
pH | 10 |
CAS Min % | 0.39 |
DOT Information | Not Regulated by DOT |
Chemical Name or Material | Buffer, Reference Standard |
CAS Max % | 0.41 |
Melting Point | 0°C |
Buffer Solution, pH 4, Color-coded Red (Certified), Fisher Chemical™ PROMO
Color-coded buffer solution made of Potassium Hydrogen Phthalate.
Boiling Point | 100°C |
---|---|
Description | Potassium Phosphate Monobasic/Sodium Hydroxide. pH/temperature conversions given on label. |
Color | Yellow |
Traceability to NIST | Traceable to NIST. |
Physical Form | Liquid |
Chemical Name or Material | Buffer Solution, pH 7.00, Color-Coded Yellow |
Grade | Certified |
Identification | Pass Test |
ChemAlert Storage Symbol | Gray |
Density | 1.0g/cm³ |
Vapor Pressure | 760mmHg at 20°C |
Absorbance | Pass Test |
CAS | 1934-21-0 |
Health Hazard 3 | Emergency Overview May cause eye, skin, and respiratory tract irritation. Handle in accordance with good industrial hygiene and safety practice. Wash off immediately with plenty of water for at least 15 minutes. Get medical attention immediately if symptoms occur. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Get medical attention immediately if symptoms occur. Do not induce vomiting. Obtain medical attention. . . NFPA Health:1 Flammability:0 Instability:0 |
MDL Number | MFCD00134130 |
Health Hazard 2 | CAUTION! |
Solubility Information | Soluble in water |
pH | 7.00 |
Specific Gravity | 1 |
Melting Point | 0°C |
Buffer Solution, pH 8.00 (Certified), Fisher Chemical™
Potassium Phosphate Monobasic/Sodium Hydroxide
![EDGE](/content/dam/fishersci/glyphs/edge.png)
CAS | 7732-18-5 |
---|---|
Health Hazard 3 | Emergency Overview Irritating to eyes and skin. The toxicological properties have not been fully investigated. Use personal protective equipment. Ensure adequate ventilation. Wash off immediately with plenty of water for at least 15 minutes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Get medical attention immediately if symptoms occur. Do not induce vomiting. Do not induce vomiting. Obtain medical attention. NFPA Health:2 Flammability:0 Instability:0 |
MDL Number | MFCD00147253 |
Health Hazard 2 | WARNING! |
Traceability to NIST | Traceable to NIST. |
Physical Form | Liquid |
pH | 8.0 |
Chemical Name or Material | Buffer Solution, pH 8.00 |
Grade | Certified |
Identification | Pass Test |
ChemAlert Storage Symbol | Gray |
HEPES Buffer, 1M Solution, pH 7.3 (Molecular Biology), Fisher BioReagents™
Commonly used buffering agent
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Color | Undesignated |
---|---|
Physical Form | Liquid |
Chemical Name or Material | HEPES Buffer |
Grade | Molecular Biology |
Identification | Pass Test |
DNase | DNase free |
Merck Index | 15, 4689 |
Concentration | 0.95 to 1.05 M |
ChemAlert Storage Symbol | Gray |
Absorbance | ≤0.01(0.1M solution) at 260nm,0.004 max. (0.1M solution) at 280nm |
Name Note | 1M Solution, pH 7.3 |
CAS | 7732-18-5 |
Protease | Protease free |
pH | 7.2 to 7.5 |
Synonym | N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid |
Purity Grade Notes | DNase-, RNase- and Protease-Free |
Recommended Storage | RT |
Molecular Formula | C8H18N2O4S |
Formula Weight | 238.197 |
TAE Buffer, Tris-Acetate-EDTA, 50X Solution, Electrophoresis, Fisher BioReagents™
Tris-Acetate-EDTA, CAS Number-77-86-1, 60-00-4, 6850-28-8, TAE, 4L, Gray, Tris (24%), Acetic Acid (5.0%), and EDTA (<2%)., DNase free, Pass Test, Filtered through a 0.2-micron filter., Electrophoresis, 50X Solution, Poly CUBE, Liquid, Protease free, DNase-, RNase- and Protease-Free, RT
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Name Note | 50X Solution |
---|---|
Concentration or Composition (by Analyte or Components) | Tris (24%), Acetic Acid (5.0%), and EDTA (<2%). |
CAS | 6850-28-8 |
Protease | Protease free |
Physical Form | Liquid |
Grade | Electrophoresis |
Synonym | TAE |
Purity Grade Notes | DNase-, RNase- and Protease-Free |
DNase | DNase free |
Recommended Storage | RT |
Filtered Through | Filtered through a 0.2-micron filter. |
ChemAlert Storage Symbol | Gray |
Buffer Solution, pH 10, Color-coded Blue (Certified), Fisher Chemical™ PROMO
Color-coded buffer solution for research use.
CAS | 7732-18-5 |
---|---|
MDL Number | MFCD00146206 |
Chemical Name or Material | Buffer Solution, pH 1.01 |
Grade | Certified |
Linear Formula | KHC8H4O4 |
---|---|
Color | Red |
Traceability to NIST | Traceable to NIST |
Physical Form | Liquid |
Chemical Name or Material | Buffer Solution pH 4.0 at 25°C |
Grade | Certified |
Identification | Passes Test |
Density | 1g/mL |
Name Note | Color Coded Red |
CAS | 67-56-1 |
Health Hazard 3 | GHS P Statement If in contact with skin or eyes, rinse thoroughly with water for 15-20 minutes. If swallowed, get medical attention. |
Decomposition Information | Formaldehyde; Carbon monoxide; Carbon dioxide |
Health Hazard 2 | GHS H Statement Solution is not hazardous. |
Solubility Information | Soluble in water |
pH | 4.00 to 4.01 |
Recommended Storage | Room Temperature |
Formula Weight | 204.23 |
Specific Gravity | 1g/mL |
CAS Max % | 0.005 |
Buffer pH 4.00 ± 0.01 at 25°C, Color-Coded Red Reference Standard, Reagents
Buffer pH 4.00 ± 0.01 at 25°C, Color-Coded Red Reference Standard, Reference Buffer, use for pH meter calibration. Manufactured in ISO 9001 facility. Accredited to ISO 17025, NIST Traceable.
Boiling Point | 100°C |
---|---|
CAS | 877-24-7 |
For Use With (Equipment) | pH Meter |
Color | Pink |
Physical Form | Liquid |
pH | 4 |
Chemical Name or Material | Buffer pH 4.00 ± 0.01 at 25°C, Color-Coded Red Reference Standard |
For Use With (Application) | Calibration |
Sterility | Non-sterile |
Specific Gravity | 1.0039 |
Solution Type | Buffer |
Melting Point | 0°C |
VeriSpec™ pH Buffer, Certified Reference Material
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ISO 17025/17034 pH Buffer Certified Reference Materials provide the highest level of quality assurance. A certificate of analysis providing the specified property value, uncertainty, and traceability is available with every bottle.
For Use With (Equipment) | pH Meter and Electrode |
---|---|
Formulation | Aqueous Solution |
Traceability to NIST | NIST Traceable |
Physical Form | Liquid |
Certificate of Analysis | Yes |
Solution Type | pH Buffer |
Product Line | VeriSpec |
Linear Formula | KH2PO4 |
---|---|
Color | Colorless |
Traceability to NIST | Traceable to NIST |
Physical Form | Liquid |
Chemical Name or Material | Buffer Solution pH 7.0 at 25°C |
Grade | Certified |
Identification | Passes Test |
CAS | 1310-73-2 |
Health Hazard 3 | GHS P Statement If in contact with skin or eyes, rinse thoroughly with water for 15-20 minutes. If swallowed, get medical attention. |
Decomposition Information | Phosphorus oxides; Sodium oxide |
Health Hazard 2 | GHS H Statement Solution is not hazardous. |
Solubility Information | Soluble in water |
pH | 7.00 ±0.01 |
Recommended Storage | Room Temperature |
Formula Weight | 136.09 |
CAS Max % | 0.08 |