Test
For enzyme immunoassay and immunohistochemistry
Water-soluble compound used to biotinylate cell-surface proteins and proteins that are sensitive to organic solvents
Sulfur-containing vitamin that plays role in numerous carboxylation and deamination reactions
For labeling antibodies, hormones or other polypeptide molecules containing lysine residues
Avidin/Biotin Blocking System Apps: IHC, WB; Size: 2 x 15 ml
Secondary Streptavidin-HRPSecondary Streptavidin-HRP Conjugate (ready-to-use reagent). For Wes, Sally Sue, and Peggy Sue (2 x 1 mL). Use with a biotinylated antibody. For total protein assay use 042-976 and to detect biotinylated molecular weight ladders use 042-414. Ships with ice pack. Upon receipt, store at 2-8°C.
Tris-NTA Biotin, 1 mg/ml, 0.1mg
Biotin (Long Arm) Maleimide is designed for biotinylating proteins, nucleic acids, or other molecules containing one or more thiol groups. It reacts under mild acid or neutral pH conditions with free sulfhydryl groups such as those of cysteine residues or sulfhydryls introduced chemically into proteins or other macromolecules. A 6-aminohexanoate spacer arm between the maleimide group and biotin reduces the possibility of steric hindrance and ensures that the biotin is fully accessible to avidin or streptavidin detection systems. The extraordinary affinity of avidin and streptavidin to biotin and the many biotin-avidin/streptavidin systems available make the biotin label ideal for a variety of applications including in situ hybridization, blotting, and affinity binding.
biotin conjugation kit fast type b lightning link
Amine-reactive, medium length biotinylation agent
Biotin is a small biomolecule important for many cellular processes. Most importantly for biotechnology applications, biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptavidin; an attraction that is the strongest and most stable non-covalent interaction known.Beta-galactosidase (beta-gal; β-gal) is an enzyme that hydrolyzes β-galactosides (such as lactose and various glycoproteins) into simple sugars. Beta-galactosidase is routinely used as a reporter in biochemical experiments as well as a marker for gene expression experiments in E. coli. Beta-galactosidase can be used to cleave the chemical X-gal that forms an intense blue product that can easily be quantified.
Streptavidin Poly-HRP Conjugate is streptavidin biotin-binding protein that is conjugated with polymers of horseradish peroxidase, enabling signal amplification and detection of biotinylated antibodies for IHC and other methods. This poly-HRP conjugate is designed to deliver the highest sensitivity and low background in immunoassays where sample volume is limited or when the target molecule is present at low levels. The estimated average number of HRP monomer molecules in SA-PolyHRP20 conjugate is 100 (20 X 5), in SA-PolyHRP40 - 200 (40 X 5) and in SA-PolyHRP80 - 400 (80 X 5). Thus, PolyHRP brings in reaction with substrate development system much larger number of enzyme label molecules (per one bound analyte molecule) than conventional conjugates do.