Test
Digest human or mouse IgG antibodies with immobilized papain protease and purify Fab fragments using Protein A agarose.
Selectively digest whole human and other IgG antibodies with immobilized pepsin protease to make F(ab')2 fragments that retain antigen binding activity.
Digest human or mouse IgG antibodies with immobilized papain protease and purify Fab fragments using Protein A agarose.
Efficiently digest mouse IgG1 antibodies into Fab or F(ab')2 fragments with immobilized ficin protease.
Selectively digest whole human and other IgG antibodies with immobilized pepsin protease to make F(ab')2 fragments that retain antigen binding activity.
Efficiently digest mouse IgG1 antibodies into Fab or F(ab')2 fragments with immobilized ficin protease.
NC1664638 FRAGIT KIT DIGEST/PUR 10X0.5MG
Immobilized Pepsin resin (Pepsin Agarose) is commonly used to create F(ab’)2 fragments from antibodies. By immobilizing pepsin autolysis and protease contamination is essentially eliminated. Immobilized Pepsin gel hydrolyzes protein non-specifically at pH 1.0 to 3.0, with an optimum activity at pH 1.0. ProteoChem's highly active pepsin is covalently bound to crosslinked 6% beaded agarose resin and its specific activity is > 6,100 units/ml resin (one unit is defined as releasing 0.001 A280 as TCA hydrolyzed/min. at 37°C). ProteoChem's Immobilized Pepsin is packaged as a slurry in buffer at pH 4.5 containing 50% glycerol, 100 mM Sodium Acetate, 0.05% NaN3.
VersaComp Antibody Capture Bead kit provides both positive and negative microspheres that can be used to set compensation for multicolor flow cytometry experiments. The positive population captures conjugates in single color stains and recognizes all mouse and rat isotypes, most hamster isotypes and rabbit polyclonal IgG. The negative population provides fluorescence similar to unstained cells across the different excitation/emission wavelength combinations. This combination can be used to set up compensation to correct for spectral overlap.
NC1458861 FRAGIT KIT MICROSPIN 0.5MG
NC0919531 FRAGIT MICROSPIN CLEAVES 2 X
5 FragIT MicroSpin columns + 5 Fc fragment affinity columns. FragIT kit is an extremely rapid and easy way of creating and isolating F(ab')2 and Fc fragments from IgG. The kit consist of two spin columns - one for fragmentation (FragIT column) and one for purification of F(ab')2 and isolation of Fc fragments. The FragIT column is FabRICATOR enzyme immobilized on agarose and the second column has affinity ligands selective for Fc fragments.
NC0983557 FRAGIT MICROSPIN CLEAVES 5X0.5
NC1240627 FABULOUS FAB KIT MOUSE 2MG IGG