Direct PCR Reagents and Kits
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Direct PCR Reagents and Kits
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Filtered Search Results
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Thermo Scientific™ Phire Plant Direct PCR Master Mix
Amplify DNA directly from plant samples without prior DNA purification with Phire Plant Direct PCR Master Mix
Polymerase | Phire Hot Start II DNA polymerase |
---|---|
GC-Rich PCR Performance | Medium |
Reaction Format | SuperMix or Master Mix |
Product Type | Direct PCR Master Mix |
Sample Type | Plant |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Starting Material | DNA |
For Use With (Application) | Genotyping |
PCR Method | Direct PCR |
Product Line | Phire |
Invitrogen™ Platinum™ Direct PCR Universal Master Mix
Invitrogen Platinum Direct PCR Universal Master Mix is designed to amplify DNA directly from various samples without the need to purify DNA.
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Shipping Condition | Dry Ice |
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Polymerase | Platinum II Taq Hot-Start DNA Polymerase |
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 1X |
Size (Final Product) | 8 kb |
Reaction Format | SuperMix or Master Mix |
Product Type | Direct PCR Universal Master Mix |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | 3'-A |
Green Features | Less Hazardous, Less Waste |
Concentration | 2X |
For Use With (Application) | Hot-start PCR,Real Time PCR (qPCR) |
Thermo Scientific™ Phire Animal Tissue Direct PCR Kit (without sampling tools)
PCR directly from various animal tissues including mice, fish, birds and insects without DNA purification.
Invitrogen™ PureLink™ PCR Purification Kit
The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits.
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Shipping Condition | Room Temperature |
---|---|
Format | Kit |
Sample Type | PCR Products |
Isolation Technology | Silica Spin Column |
Run Time | 10 min. |
Thermo Scientific™ Phire Plant Direct PCR Kit (without sampling tools)
Efficient PCR directly from different plant materials across species.
Invitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit
Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step TaqMan™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.
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GC-Rich PCR Performance | High |
---|---|
Format | Tube |
Product Type | 1-Step qPCR kit |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
Sample Type | Cell, RNA |
Reaction Speed | Fast or Standard |
Reagent Type | Cell Lysis |
Green Features | Fewer resources used and less waste, Less hazardous |
For Use With (Application) | Gene Expression |
PCR Method | 1-step RT-qPCR |
Product Line | Cells-to-CT™, TaqMan™ |
Detection Method | Primer-probe |
Thermo Scientific™ Phusion Blood Direct PCR Kit
Perfrom PCR directly from whole blood without DNA purification.
Polymerase | Phusion Blood II DNA Polymerase |
---|---|
GC-Rich PCR Performance | High |
Reaction Format | Separate Components |
Product Type | Direct PCR Kit |
Sample Type | Blood |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Starting Material | DNA |
PCR Method | Direct PCR |
Product Line | Phusion |
Invitrogen™ Cells-to-cDNA™ II Kit
The Ambion™ Cells-to-cDNA™ II Kit (patent pending) produces cDNA from cultured mammalian cells in less than 2 hr., no RNA isolation is required.
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Shipping Condition | Dry Ice |
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Content And Storage | 1X PBS, Cell Lysis II Buffer, μL DNase 1, 10X RT Buffer, M-mLV Reverse Transcriptase, RNase Inhibitor, dNTP Mix, Random Decamers, Oligo(dT)18 Primers, Armored RNA™ Control, Armored RNA™ Primer Pair, and Endogenous Primer Pair should be stored at -20°C. Nuclease-free water may be stored at any temperature. |
Reverse Transcriptase | M-MLV |
Size (Final Product) | 7 kb or less |
Final Product Type | cDNA |
Sample Type | Mammalian Cells |
Purification Time | 2 hr. |
Optimal Reaction Temperature | 42°C |
For Use With (Application) | Real Time PCR (qPCR),RT-PCR |
Product Line | Ambion™, Cells-to-cDNA™ |
Applied Biosystems™ MagMAX™ Viral/Pathogen Binding Beads
These binding beads are included in the MagMAX Viral/Pathogen Nucleic Acid Isolation kits and MagMAX Microbiome Ultra Nucleic Acid Isolation Kit.
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Thermo Scientific™ GeneJET PCR Purification Kit
Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.
Format | Spin Column |
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Run Time | 5 min. |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Invitrogen™ ChargeSwitch™ PCR Clean-Up Kit
ChargeSwitch PCR Clean-Up Kit is the fastest and most effective method of removing primers, unincorporated dNTPs, thermostable polymerases and salts.
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Shipping Condition | Room Temperature |
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Target | DNA from Enzymatic Reactions (e.g. PCR) |
Content And Storage | The ChargeSwitch™ PCR Clean-Up Kit is shipped at room temperature. Upon receipt, store all components at room temperature. All components are guaranteed stable for 6 months when stored properly. |
Format | Kit |
Product Type | PCR Clean-Up Kit |
Sample Type | PCR Products |
Isolation Technology | ChargeSwitch™ Chemistry |
Run Time | 5 min |
For Use With (Application) | Next-Generation Sequencing (NGS),Cloning,Standard PCR |
High-throughput Compatibility | High-throughput Compatible |
Product Line | ChargeSwitch™ |
Thermo Scientific™ Phire Tissue Direct PCR Master Mix
Amplify DNA directly from variety of human and animal samples with no prior purification with Phire Tissue Direct PCR Master Mix.
Polymerase | Phire Hot Start II DNA polymerase |
---|---|
GC-Rich PCR Performance | Medium |
Includes Label or Dye | Yes |
Reaction Format | SuperMix or Master Mix |
Product Type | Direct PCR Master Mix |
Sample Type | Tissue |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Starting Material | DNA |
For Use With (Application) | Genotyping |
PCR Method | Direct PCR |
Product Line | Phire |
Invitrogen™ GeneArt™ Genomic Cleavage Detection Kit
The GeneArt Genomic Cleavage Detection Kit provides a simple, reliable, and rapid method for the detection of locus-specific double-strand break formation.
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Invitrogen™ PureLink™ PCR Micro Kit
PureLink PCR Micro Kits are ideal for purifying and concentrating DNA from low-yielding PCR or restriction digestion reactions.
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Shipping Condition | Room Temperature |
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Separation Range | 100 bp to 12 kb |
Content And Storage | Kit includes columns, binding and wash buffers, recovery tubes, and the manual. Store at room temperature. |
Starting Material Volume | 125 μL |
Isolation Technology | Silica Spin Column |
Elution Volume Max | 20 μL |
For Use With (Application) | Cloning,Standard PCR |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Target | DNA from Enzymatic Reactions (e.g. PCR) |
Format | Spin Column |
Product Type | PCR Micro Kit |
Run Time | 10 min. |
Elution Volume Min | 5 μL |
Product Line | PureLink™ |
Invitrogen™ PureLink™ Pro 96 PCR Purification Kit
The PureLink™ Pro 96 PCR Purification Kit combines advanced silica plate extraction chemistry with an optimized 96-well plate design for manual or automated processing of PCR cleanup in as little as 20 minutes.
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