Thermo Scientific™ SureTect™ Listeria monocytogenes PCR Assay

Rapidly detect Listeria species monocytogenes in food and environmental samples with the Thermo Scientific™ SureTect™ Listeria Species monocytogenes PCR Assay.

Supplier:  Thermo Scientific™ A56843

Catalog No. A56843

Description

Detect Listeria monocytogenes in even the most challenging food types with the Thermo Scientific™ SureTect™ Listeria monocytogenes PCR Assay, which incorporates optimized primer and probe components for highly-specific detection of L. monocytogenes.

The assay's workflow holds AOAC Performance Tested Methods^SM approval granted by the AOAC Research Institute and is an AOAC Official Method^SM, with First Action status granted by AOAC International. The assay is also validated according to ISO 16140-2:2016 and certified by NF Validation™ by AFNOR Certification. The assay is certified to test a broad range of foods and environmental samples.

The SureTect System is an optimized solution combining speed and performance in an easy-to-use, cost-effective platform—giving you results you can trust.

The new SureTect assay format incorporates color-coded plastics with orientation markings and breakable strip format, colored reagents, and pierceable seals to enable an optimized workflow for maximum laboratory efficiency and productivity.

Simple

• Easy-to-use with single enrichment step and pre-dispensed reagents
• Target-specific, colored assay plastics
• Clearly oriented lysis and assay trays
• Color indicator in lysis reagent reducing possibility for human error

Fast and Flexible

• Option to automate the lysis and PCR sample preparation
• Optimized workflow delivers on-screen results in as little as 22 hours
• Maximum flexibility for operational efficiency
• Run all assays simultaneously in the same run
• Test up to 96 samples in each run
• Choice of pierceable seals or caps
• Detect multiple targets in the same run with universal PCR protocols across the range 

Smart

• Intuitive, user-friendly software delivers reliable results, with no subjective interpretation
• Actionable data supports fast decisions in a complex product supply chain
• Internal positive control (IPC) in every assay for complete confidence in results

The Thermo Scientific SureTect Listeria monocytogenes PCR Assay Kit includes:

• Lysis Reagent 1 Tubes
• Lysis Reagent 2 
• Proteinase K
• Listeria monocytogenes PCR Assay Tubes
• Thermo Scientific 96-Well Pierceable Seals
• PCR Caps

Enriched samples are pipetted into pre-filled Lysis Tubes, along with Proteinase K and Lysis Reagent 2, before incubation to lyse any bacterial cells present in the sample and release their DNA into the solution. The lysate is then loaded into the SureTect Listeria monocytogenes PCR Tube to re-hydrate the preloaded PCR pellet which contains all of the necessary components and reagents for PCR, including a probe, primers and DNA template for the internal positive control (IPC). 

The PCR tubes are then sealed, loaded into the Applied Biosystems™ QuantStudio™ 5 Food SafetyReal-Time PCR System used with RapidFinder™ Analysis software v3.0 (or later) or the Applied Biosystems™ 7500 Fast Food Safety 96-well Real-Time PCR instrument used with the RapidFinder™ Express Software v2.0 or later. After running, interpreted results are clearly displayed and can be reported, stored, printed, or downloaded as required.

Specifications

• No. of Tests: Test up to 96 samples
• Product Type: SureTect Listeria moncytogenes
• For Use With (Application): Food & Beverage

• Product Line: SureTect
• Target Organism Class: Listeria monocytogenes