Thermo Scientific™ Remel™ BactiDrop™ Spot Indole

Determine the ability of microorganisms to split indole from tryptophan molecule by spot test method with BactiDrop™ Spot Indole.

Supplier:  Thermo Scientific™ R21550

Catalog No. R21550

Description

Determine the ability of an organism to split indole from the tryptophan molecule with Thermo Scientific™ Remel™ BactiDrop™ Spot Indole. Vracko and Sherris, in 1963 used indole-spot reagent for the presumptive separation of Proteus species and Escherechia coli1. In 1969, Lowrance, Reich and Traub found ρ-dimethylaminocinnamaldehyde (DMACA) to be the most sensitive indole reagent which could detect 3 mcg of indole per milliliter of medium2.

BactiDrop™ Spot Indole is used to determine the ability of microorganisms to split indole from the tryptophan molecule by the spot test method. Tryptophanases are intracellular enzymes that mediate indole production by hydrolysing the amino acid tryptophan. This indole combines with DMACA to form a blue-green compound. This is a condensation product formed by an acid splitting of the protein.Ready to use: Liquid reagentRapid test: Results within 1-3 minutesEasy to interpret: Positive results (blue color) are easy to interpret from negative results (pink color).BactiDrop™ Spot Indole Reagent contains 1% w/v ρ-dimethylaminocinnamaldehyde (CAS 6203-18-5) in hydrochloric acid (concentrated) (CAS 7647-01-0) and demineralized water (CAS 7732-18-5). The reagent is supplied as 0.75 mL ampules.Not all products are available for sale in all territories. Please inquire.Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.

Specifications

• Content And Storage: 20°C to 25°C
• CE Marker: Yes
• Quantity: 50 x 0.75 mL

• Description: BactiDrop Spot Indole
• Detectable Analytes: Nitrite, Free Nitrogen Gas