Rat anti-Mouse IgG2a Secondary Antibody, Super Bright™ 436, eBioscience™, Invitrogen™

Rat Monoclonal Secondary Antibody

Catalog Number	Mfr. No.	Quantity
62-421-082	eBioscience 62421082	100μg

Description

Description: The monoclonal antibody m2a-15F8 recognizes Mouse IgG2a antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG2a or any rat isotype antibodies. Applications Reported: This m2a-15F8 antibody has been reported for use in flow cytometric analysis. Applications Tested: This m2a-15F8 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at less than or equal to 1.0 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product No. SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Mouse IgG2a
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Isotype: IgG1 κ
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!

• Clone: m2a-15F8
• Applications: Flow Cytometry
• Conjugate: Super Bright 436
• Host Species: Rat
• Target Species: Mouse
• Primary or Secondary: Secondary