Thermo Scientific™ Pierce™ ECL Plus Western Blotting Substrate

Acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.

Supplier:  Thermo Scientific™ 32132

Catalog No. FER32132

Description

Thermo Scientific™ Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated probes by Western blotting using X-ray film, CCD or laser-based imagers.

Pierce ECL Plus Substrate is a chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated antibodies and probes by Western blotting using X-ray film, CCD camera documentation systems or laser-based imagers. Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for Western blotting.

Highlights:

• Easy to use — can be substituted for the discontinued GE ECL Plus Substrate without any re-optimization

• Higher sensitivity — detect targets down to the low-picogram level

• Longer signal duration — sustained light output for as long as five hours

• More imaging options — X-ray, CCD or laser-based imagers

• More affordable — high quality and performance at a lower price than other suppliers« similar substrates

Specifications

• Description: Pierce ECL Plus Western Blotting Substrate
• Detection Method: Chemiluminescent, Fluorescent
• For Use With (Application): Protein Assay and Analysis
• Product Line: Pierce™
• Conjugate: HRP
• Sufficient For: 10 mini-gel blots; 1000cm2 of membrane
• Content And Storage: 2°C to 8°C

• Product Type: Substrate
• Form: Liquid
• Membrane Compatibility: Nitrocellulose, PVDF
• Quantity: 100 mL Kit
• Sensitivity: Low picogram to mid-femtogram
• Kit Contents: Detection Reagent A, 100mL, Detection Reagent B, 2.5mL

For Research Use Only. Not for use in diagnostic procedures.