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Thermo Scientific™ Pierce™ ECL Plus Western Blotting Substrate
Acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.
Supplier: Thermo Scientific™ 32132
Description
Thermo Scientific™ Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated probes by Western blotting using X-ray film, CCD or laser-based imagers.
Pierce ECL Plus Substrate is a chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated antibodies and probes by Western blotting using X-ray film, CCD camera documentation systems or laser-based imagers. Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for Western blotting.Highlights:
Easy to use — can be substituted for the discontinued GE ECL Plus Substrate without any re-optimization
Higher sensitivity — detect targets down to the low-picogram level
Longer signal duration — sustained light output for as long as five hours
More imaging options — X-ray, CCD or laser-based imagers
More affordable — high quality and performance at a lower price than other suppliers« similar substrates
Specifications
Pierce ECL Plus Western Blotting Substrate | |
Chemiluminescent, Fluorescent | |
Protein Assay and Analysis | |
Pierce™ | |
HRP | |
10 mini-gel blots; 1000cm2 of membrane | |
2°C to 8°C |
Substrate | |
Liquid | |
Nitrocellulose, PVDF | |
100 mL Kit | |
Low picogram to mid-femtogram | |
Detection Reagent A, 100mL, Detection Reagent B, 2.5mL |
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For Research Use Only. Not for use in diagnostic procedures.