Thermo Scientific™ iodoTMTsixplex™ Isobaric Mass Tagging Kit

Use Iodoacetyl Tandem Mass Tag™ (iodoTMT™) Isobaric Label Reagents to label up to six sulfhydryl-containing peptides for simultaneous quantitation of proteins using tandem mass spectrometry.

Supplier:  Thermo Scientific™ 90103

Catalog No. PI90103

Description

Thermo Scientific iodoTMTsixplex Isobaric Mass Tag Labeling Kit contains iodoTMT™ reagents and associated materials for the irreversible labeling of sulfhydryl groups. Iodoacetyl Tandem Mass Tag™ Reagents enable selective labeling of cysteine-containing peptides for multiplexed sample quantitation using LC-MS/MS analysis.

The iodoTMT Reagents are sets of isobaric (mass and structure) isomers that are iodoacetyl-activated for covalent, irreversible labeling of sulfhydryl (—SH) groups. Similar to iodoacetamide, iodoTMT Reagents react specifically with reduced cysteines (Cys) in peptides and proteins and enable quantitation of the relative abundance of cysteine modifications, such as S-nitrosylation and the oxidation of disulfide bonds.

The Thermo Scientific iodoTMTzero Label Reagent allows testing and optimization of sample preparation, labeling, fractionation and MS fragmentation for peptide identification and reporter detection without using the more costly isotope-labeled compounds.

Features of the iodoTMT Reagents:
• Specific—only reacts with sulfhydryl groups (reduced, unmodified cysteine residues)
• Irreversible—labeled proteins and peptides are not susceptible to reducing agents
• Flexible—options for duplex isotopic (MS) or sixplex isobaric (MS/MS) quantitation
• Versatile—can be used to study cysteine modifications (e.g. di-sulfide bridges, S-nitrosylation)

To selectively analyze the cysteine-labeled peptides, the sample may be enriched using the Thermo Scientific Immobilized Anti-TMT Antibody Resin (Cat. No. 90076) and TMT Elution Buffer (Cat. No. 90104). This approach of selective thiol-labeling and affinity enrichment is similar to isotope-coded affinity tags (ICAT™ Method) but allows for affinity enrichment with a non-biological tag and higher multiplex quantitation.

Specifications

• Description: iodoTMTsixplex™ Isobaric Mass Tagging Kit
• Product Line: iodoTMTsixplex™
• Label or Dye: Tandem Mass Tag™ (TMT) Labels
• Final Product Type: Protein
• Workflow Step: Protein Labeling

• For Use With (Equipment): Mass Spectrometer
• Content And Storage: • iodoTMT6-126 Label Reagent, 5 x 0.2 mg
  • iodoTMT6-127 Label Reagent, 5 x 0.2 mg
  • iodoTMT6-128 Label Reagent, 5 x 0.2 mg
  • iodoTMT6-129 Label Reagent, 5 x 0.2 mg
  • iodoTMT6-130 Label Reagent, 5 x 0.2 mg
  • iodoTMT6-131 Label Reagent, 5 x 0.2 mg
  • HES Buffer, 15 mL
  • Bond-Breaker TCEP Solution, 0.5 mL
  • DTT, No-Weigh Format, 8 x 7.7 mg
  • Trypsin, 5 x 20 μg
  • Trypsin Storage Solution, 250 μL
  • Albumin (bovine), 2.5 mg
  
  Upon receipt store all products at –20°C
  
  
• Starting Material: Cell Lysate
• Detection Method: Mass Spectrometry
• Quantity: 30 Reactions

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.