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Thermo Scientific™ BS(PEG)9 (PEGylated bis(sulfosuccinimidyl)suberate)
Crosslink amines to amines with these long (21.7A and 35.8A) crosslinkers composed of NHS-ester groups and 5-unit or 9-unit PEG spacer arms that enhance protein solubility.
Supplier: Thermo Scientific™ 21582
Description
Thermo Scientific™ Pierce BS(PEG)n Crosslinkers include BS(PEG)5 and BS(PEG)9, which are bis-succinimide ester-activated PEG compounds for crosslinking between primary amines in proteins and other molecules.
BS(PEG)9 is a bis-succinimide ester-activated PEG compound used for crosslinking between primary amines (–NH2) in proteins and other molecules. The N-hydroxysuccinimide ester (NHS) groups at either end of the PEG9 spacer react specifically and efficiently with lysine and N-terminal amino groups at pH 7-9 to form stable amide bonds.
Features of BS(PEG)9:
- Reactive groups: NHS ester (both ends)
- Reactive towards: amino groups (primary amines)
- NHS esters react with –NH2 groups at pH 7-9, forming stable amide bonds
- Water-soluble; compare to non-pegylated BS3 (also soluble) (Cat. No. 21580) and DSS (water-insoluble) (Cat. No. 21655)
- Membrane-impermeable, allowing for cell surface labeling
- Irreversibly crosslink proteins or peptides by flexible PEG spacer arms
- Polyethylene glycol spacer arms help maintain conjugate solubility
- Pure compound with defined structure and molecular weight, ensuring reproducible protein-modification effects
- PEG spacer provides unique advantages, including increased stability, reduced tendency toward aggregation and reduced immunogenicity
- Ideal for small molecule or peptide conjugations
Alternative Names for BS(PEG)n:
- Bis-NHS-dPEG
- NHS-PEG-NHS
- BS(PEO)5
- Bis(NHS)PEO5
- BS-PEO9
- dPEG NHS ester
- Bis-succinimide ester PEG 400
Product References
- Crosslinker Application Guide -- search for recent literature references for this product
- Bioconjugate Techniques, Academic Press (Cat. No. 20036)
Related Products
- BS(PEG)5 (PEGylated bis(sulfosuccinimidyl)suberate) (Cat. No. 21581)
- BS3 (bis(sulfosuccinimidyl)suberate) (Cat. No. 21580)
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Specifications
Amine-Amine | |
BS(PEG)9 | |
Yes | |
Upon receipt store desiccated at -20°C. | |
Ambient | |
Chemical Labeling | |
NHS Ester | |
Liquid | |
Water | |
Yes |
No | |
708.71 | |
35.8 Å | |
No | |
Pierce™ | |
Homobifunctional | |
Long (>30 Å) | |
100 mg | |
Standard | |
Crosslinker |
Safety and Handling
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For Research Use Only. Not for use in diagnostic procedures.