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MilliporeSigma™ Novagen™ Overnight Express™ Autoinduction NMR Medium-15N
Provides reagents to make a chemically defined autoinducing liquid culture medium used to produce [U-15N] labeled proteins for NMR spectroscopy. Novagen™ Overnight Express™ Autoinduction NMR Medium- 15N system includes four concentrated sterile solutions.
$2150.00
Specifications
Content And Storage | Multiple storage requirements. Ok to freeze. |
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Product Type | NMR Medium-15N |
Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
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Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
71-759-4
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MilliporeSigma™
717594 |
5 L Kit |
Each for $2,150.00
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71-759-3
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MilliporeSigma™
717593 |
1 L Kit | Item Discontinued This item has been discontinued by the Fisher Scientific channel. Please Sign In to view product availability in your area. | N/A | |||||
Description
- Autoinduction method is based on the differential metabolism of carbon sources to promote growth to high cell density
- Automatically induce protein expression under control of lac promoters, eliminating the need to monitor cell densities or add inducer (IPTG)
- Includes four concentrated sterile solutions:
- OnEx NMR Solution 1 is a blend of carbon sources optimized for tightly regulated uninduced growth to high density, followed by autoinduction
- OnEx NMR Solution 2 is a buffer and nitrogen blend that mediates metabolic acid production and provides additional nitrogen for increased protein synthesis
- OnEx Solution 3 provides magnesium for maximal cell density
- OnEx Solution 4 provides trace metals to minimize growth limitations associated with mineral deficiencies and satisfy the requirements of metal-containing target proteins, even at high expression levels
Specifications
Multiple storage requirements. Ok to freeze. | |
NMR Medium-15N |
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