Abnova™ MDM2 CISH Probe

MDM2 CISH Probe is designed for the qualitative detection of human MDM2 gene in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).

Supplier:  Abnova™ CG0020

Catalog No. 89-335-567

Description

The probe is intended to be used in combination with the CISH Implementation Kit 1, which provides necessary reagents for specimen pretreatment and post-hybridization processing.

Hybridization signals of digoxigenin-labeled polynucleotides appear as brown- to dark brown colored distinct dots (MDM2 gene region).
Normal situation: In interphases of normal cells or cells without an amplification involving the MDM2 gene locus, two distinct dot-shaped brown signals per nucleus appear.
Aberrant situation: In a cell with amplification of the MDM2 gene locus or polysomy of chromosome 12, multiple copies of the brown signal or brown signal clusters will be observed. Other signal distribution may be observed in some abnormal samples which might result in a different signal pattern than described above, indicating variant rearrangements.
Unexpected signal patterns should be further investigated.

Specifications

• Includes: Reagent Provided:
  
  1. Digoxigenin-labeled polynucleotides targeting sequences mapping in 12q15* (chr12:69,190,708-69,430,185) harboring the MDM2 gene
  2. Formamide based hybridization buffer
  
  *according to Human Genome Assembly GRCh37/hg19
• Content And Storage: The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18 to 25°C) and mix briefly before use.
• For Use With (Application): CISH-P