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Invitrogen™ Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)
Invitrogen Lyo-ready RPA reagents are designed for fast, sensitive, and specific DNA amplification through isothermal recombinase polymerase amplification (RPA) reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats.
Supplier: Invitrogen™ A72128
Description
Invitrogen Lyo-ready recombinase polymerase amplification (RPA) reagents are designed for fast, sensitive, and specific DNA amplification through isothermal RPA reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats. Reverse transcriptase can be added to the RPA reaction for amplification of RNA targets. All reagents are glycerol-free, which enables assay lyophilization.
Features of Lyo-ready RPA products
- Enhanced sensitivity down to 1 copy using optimized (RT-)RPA reaction buffer
- Robust and specific RNA/DNA amplification
- Amplification from inhibitor-containing samples
- Fast target amplification in as little as 20 minutes
- Ability to easily optimize RPA or RT-RPA reactions
Kit components
The Lyo-ready RPA Kit includes all required components for an RPA reaction:
- Lyo-ready T4 UvsX Protein—produced from coli cells bearing cloned uvsX gene derived from bacteriophage T4
- Lyo-ready T4 UvsY Protein—a recombination mediator protein that stimulates homologous recombination performed by the UvsX recombinase
- Lyo-ready Bst DNA Polymerase (Cat. No. A56655)—a proprietary mutant of Bst DNA Polymerase, Large Fragment, with fast reaction speed, increased sensitivity, and tolerance to inhibitors
- Lyo-ready T4 Gene 32 Protein—a single-stranded DNA binding protein produced from coli cells carrying cloned bacteriophage T4 gene 32
- Optimized reaction buffer, magnesium chloride, and dNTPs
Specifications
DNA Amplification Kit | |
RPA, RT-RPA | |
500 Reactions | |
Five boxes, each box contains: • Lyo-ready T4 UvsX Protein (1.2 mg/mL), 50 μL • Lyo-ready T4 UvsY Protein (1.2 mg/mL), 50 μL • Lyo-ready Bst DNA Polymerase (6 μg/mu;L), 50 μL • Lyo-ready T4 Gene 32 Protein (16 mg/mL), 50 μL • 2X RPA Reaction Buffer, 1000 μL • 280 mM MgCl2, 100 μL • 10 mM dNTP Mix, 50 μL Store at -25°C to -15°C. |
|
Lyo-ready RPA Kit | |
Dry Ice | |
Up to 20 min. |
Liquid | |
Lyo-ready Bst DNA Polymerase | |
Nucleic Acid Amplification | |
42°C | |
SuperScript IV Reverse Transcriptase | |
End point, real-time, lateral flow |
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For Research Use Only. Not for use in diagnostic procedures.