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Invitrogen™ pBAD/His Kit
Express the protein of interest with an N-terminal tag in a tightly regulated fashion
Supplier: Invitrogen™ V43001
Includes: 20μg of each pBAD/His A, B & C vector, 20μg of pBAD/His/lacZ, 1mL of 20% L-arabinose, LMG194 and TOP10 E. coli stabs.
Description
- araBAD promoter for tightly regulated expression
- Translation initiation signals optimized for E. coli expression
- N-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin and detection with Anti-HisG Antibody
- N-terminal Xpress epitope for detection and analysis with Anti-Xpress Antibody
- Enterokinase cleavage site for removing the N-terminal tag following purification
- Three vectors are provided (A, B, and C): Each has the N-terminal tag in a different reading frame relative to multiple cloning site to simplify in-frame cloning of gene
Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
Specifications
Inducible | |
araBAD | |
None | |
Ampicillin (AmpR) | |
His Tag (6x) | |
1 kit |
Arabinose | |
Bacterial Expression Vector | |
The pBAD/His Kit includes 20 μg of each pBAD/His A, B, & C vector, 20 μg of pBAD/His/lacZ, 1 ml of 20% L-arabinose, LMG194 and TOP10 E. coli stabs. Vectors and 20% L-arabinose should be stored at -20°C. Store LMG194 and TOP10 stabs at 2 - 8°C. All components are guaranteed stable for 6 months when properly stored. | |
EK (Enterokinase) Recognition Site | |
Restriction Enzyme/MCS | |
pBAD |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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