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Invitrogen™ Gateway™ pcDNA™-DEST40 Vector
Designed for high-level, constitutive expression in variety of mammalian cell lines
Supplier: Invitrogen™ 12274015
![Encompass_Preferred](/content/dam/fishersci/glyphs/encompass_preferred.png)
Includes: All destination vectors are provided lyophilized and supercoiled.
Requires : Gateway entry clone, appropriate Gateway LR Clonase™ enzyme mix, and reaction buffer.
Description
- C-terminal V5-6x His tag for easy detection and rapid purification
- Cytomegalovirus (CMV) promoter for high-level expression
- attR sites for Gateway cloning, enabling recombination with attL-flanked fragments
- Neomycin resistance gene for stable selection
- Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli
Cloning,Constitutive Expression,Gateway Cloning,Mammalian Expression,Protein Expression,Proteins, Expression, Isolation and Analysis
![TRUSTED_SUSTAINABILITY](/content/dam/fishersci/glyphs/Sustain_Badge.png)
Specifications
Constitutive | |
CMV | |
Geneticin™ (G-418) | |
His Tag (6x), V5 Epitope Tag | |
6 μg | |
Gateway™, pDEST™ |
Transfection | |
Gateway System Destination Expression Vector | |
All destination vectors are provided lyophilized and supercoiled. | |
Gateway | |
pDEST | |
Constitutive Expression |
Safety and Handling
WARNING: Cancer - www.P65Warnings.ca.gov
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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