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Invitrogen™ BioPrime™ DNA Labeling System

Specifically designed for use in the preparation of biotinylated probes

Supplier: Invitrogen™ 18094011

Catalog No. 18-094-011

$652.00 / Each of 1

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Includes: 700μL 2.5X Random Primers Solution; 175μL 10X dNTP Mixture; 100μg Control DNA; 35μL Klenow Fragment (40U/μL); 500μL Stop Buffer; 1.25mL Distilled Water

Description

Random primers (octamers) are annealed to the denatured DNA template and extended by Klenow fragment in the presence of biotin-14-dCTP to produce sensitive biotinylated-DNA probes for use in the nonradioactive detection of DNA and RNA. With the BioPrime™ DNA Labeling System, considerable net DNA synthesis occurs resulting in a 10–40 fold amplification of the probe. Probes made with this product have been used for hybridization to Southern and northern blots, plaque and colony lifts, and for in situ hybridization to chromosome spreads.

Particularly well suited for situations in which the DNA is limited
Requires less template DNA than nick translation
Amplifies template to provide an increased amount of biotinylated probe
Labels 50 to 500ng of template DNA in one reaction

Array CGH, DNA and RNA Purification and Analysis, Gene Expression Analysis and Genotyping, Genotyping and Genomic Profiling, Northern Blotting, Nucleic Acid Gel Electrophoresis and Blotting, Southern Blotting

Order Info

Shipping Condition: Approved for shipment on Wet or Dry Ice

Specifications

Content And Storage	• 700 μl 2.5X Random Primers Solution • 175 μl 10X dNTP Mixture • 100 μg Control DNA • 35 μl Klenow Fragment (40 U/μl) • 500 μl Stop Buffer • 1.25 ml Distilled Water Store at -20°C in a non-frost-free freezer.
Label or Dye	Biotin
Labeling Target	DNA (General), Genomic DNA
Labeling Method	Direct Labeling
Product Type	DNA Labeling System
Shipping Condition	Approved for shipment on Wet or Dry Ice

Detection Method	Biotin-based
Final Product Type	Probes (Labeled DNA)
Includes Label or Dye	Yes
Product Line	BioPrime™
Quantity	30 Reactions

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