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Gibco™ Ham's F-10 Nutrient Mix

Ham's F-10 Nutrient Mixture (F-10) was designed for serum-free growth of Chinese Hamster Ovary (CHO) cells.

Supplier: Gibco™ 11550043

Catalog No. 11-550-043

$37.06 / Each of 1

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Requires : Supplementation
This item is not returnable. View return policy

Description

Ham's F-10 Nutrient Mixture (F-10) was designed for serum-free growth of Chinese Hamster Ovary (CHO) cells. F-10 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including COS-7, primary rat astrocytes, and rat prostate epithelial cells.

This Ham's F-10 is modified as follows:
With: Phenol Red, L-glutamine
Without: HEPES

The complete formulation is available.

Using Ham's F-10
Compared to other basal media, F-10 contains a wider variety of components, including zinc, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-10 has led to a variety of improved formulations, such as Ham's F-12 Nutrient Mixture (F-12). F-10 contains no proteins or growth factors. Therefore, F-10 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-10 uses a sodium bicarbonate buffer system (1.2 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Specifications

Content And Storage	Storage conditions: 2°C to 8°C (protect from light) Shipping conditions: Ambient Shelf life: 12 months from date of manufacture
Cell Type	Primary Rat Astrocytes
Concentration	1 X
Product Type	Ham's F-10 Nutrient Mixture
With Additives	High Glucose, Glutamine, Phenol Red, Sodium Pyruvate
Green Features	Sustainable packaging
Product Line	Gibco™
Shipping Condition	Room Temperature

Cell Line	CHO, COS-7, and rat prostate epithelial cells
Classification	Animal Origin-free
Form	Liquid
Sterility	Sterile-filtered
Without Additives	No HEPES
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Quantity	500 mL

Safety and Handling

ShelfLife : 12 Months From Date of Manufacture

WARNING: Cancer - www.P65Warnings.ca.gov

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