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Gibco™ Basal Medium Eagle (BME)

Basal Medium Eagle (BME) is a widely used synthetic basal medium for supporting the growth of many different mammalian cells.

Supplier: Gibco™ 21010046

Catalog No. 21-010-046

$37.74 / Each of 1

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Requires : Supplementation
This item is not returnable. View return policy

Description

Basal Medium Eagle (BME) is a widely used synthetic basal medium for supporting the growth of many different mammalian cells. BME was originally developed by Harry Eagle for HeLa cells and mouse fibroblasts, when he discovered the minimum requirements for cell growth in vitro. BME has since been used for other human lines, including WI-38 and MRC-5.

This BME is manufactured as follows:

With: Phenol Red
Without: L-glutamine, HEPES

The complete formulation is available.

BME contains eight B vitamins, the ten essential amino acids, plus cystine, tyrosine, and glutamine. There are a number of modifications of BME: Minimal Essential Medium, Glasgow's Medium and Dulbecco's Modified Eagle's Medium. This BME formulation contains Earle's salts for use in a CO₂ incubator.

BME contains no proteins, lipids, or growth factors. Therefore, BME requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). BME uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Specifications

Content And Storage	Storage conditions: 2-8°C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture
Classification	Animal Origin-free
Form	Liquid
Sterility	Sterile-filtered
Without Additives	No Glutamine, No HEPES, No Sodium Pyruvate
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Quantity	500 mL

Cell Line	HeLa, mouse fibroblasts, WI-38 and MRC-5
Concentration	1 X
Product Type	Basal Medium Eagle (BME)
With Additives	Low Glucose, Phenol Red
Green Features	Sustainable packaging
Product Line	Gibco™
Shipping Condition	Room Temperature

Safety and Handling

ShelfLife : 12 Months From Date of Manufacture

WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov

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For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

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