Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotinylation Kit

Biotinylate protein amines (e.g., lysines) with this very popular mid-length (22.4A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling.

Supplier:  Thermo Scientific™ 21435

Catalog No. PI21435

Description

Thermo Scientific™ EZ-Link Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

Highlights:

• Water-soluble analog of NHS-LC-Biotin
• Reacts with primary amines at pH 7-9 in non-amine-containing buffers such as borate, carbonate or phosphate buffers
• Negative charge of -SO₃ group keeps biotinylation localized at the cell surface
• Creates an amide bond

U.S. Patents 5,892,628 and 5,872,261.

Specifications

• Description: EZ-Link Sulfo-NHS-LC-Biotinylation Kit
• Quantity: 10 reactions
• Labeling Scale: 1 to 10 mg
• Solubility: Water
• Labeling Target: Antibodies, Proteins

• Product Type: Sulfo-NHS-LC-Biotinylation Kit
• Content And Storage: Sufficient For: 10 labeling reactions, each with 1 to 10 mg of antibody
  • EZ-Link Sulfo-NHS-LC-Biotin, 25 mg
  • PBS Pack (makes 500 mL), 1 pack
  • Zeba Spin Desalting Columns (7K MWCO), 5 mL, 10 columns
  • HABA Solution, 1 mL
  • Avidin, 10 mg
  
  Store biotin and avidin reagents at -20°C. Store remaining kit components at 4°C.
• Product Line: EZ-Link™
• Label or Dye: Biotin

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.