Gibco™ DMEM, low glucose, pyruvate, no glutamine, no phenol red

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells.

Catalog Number	Mfr. No.	Quantity
11-054-020	Gibco™ 11054020	500 mL
11-054-001	Gibco™ 11054001	1 L

Description

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of Gibco DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:
With: Low Glucose, Sodium Pyruvate
Without: L-glutamine, Phenol Red, HEPES

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Specifications

• Cell Line: HeLa, 293, Cos-7, and PC-12
• Concentration: 1 X
• Serum Level: Standard Serum Supplementation
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard

• Cell Type: Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
• Form: Liquid
• Sterility: Sterile-filtered
• Product Line: Gibco™

Safety and Handling

ShelfLife : 12 Months From Date of Manufacture

For Research Use Only. Not for use in diagnostic procedures.