Gibco™ DMEM/F-12, no glutamine

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM/F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Catalog Number	Mfr. No.	Quantity
21-331-020	Gibco™ 21331020	500 mL

Description

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM/F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of Gibco™ DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM/F-12 is modified as follows:
With: Phenol Red
Without: L-glutamine, HEPES

Gibco™ DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids and vitamins with F-12's wide variety of components.

DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Specifications

• Form: Liquid
• Without Additives: No Glutamine, No HEPES
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard

Safety and Handling

ShelfLife : 12 Months

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.