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Thermo Scientific Chemicals Bromophenol Blue

Catalog Number AAA1846909
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Quantity:
10 g
50 g
115-39-9
C19H10Br4O5S
669.96
MFCD00005875
UDSAIICHUKSCKT-UHFFFAOYSA-N
Bromphenol Blue
8272
CHEBI:59424
2,6-dibromo-4-[3-(3,5-dibromo-4-hydroxyphenyl)-1,1-dioxo-2,1$l^{6}-benzoxathiol-3-yl]phenol
C1=CC=C2C(=C1)C(OS2(=O)=O)(C3=CC(=C(C(=C3)Br)O)Br)C4=CC(=C(C(=C4)Br)O)Br
115-39-9
C19H10Br4O5S
669.96
MFCD00005875
UDSAIICHUKSCKT-UHFFFAOYSA-N
Bromphenol Blue
8272
CHEBI:59424
2,6-dibromo-4-[3-(3,5-dibromo-4-hydroxyphenyl)-1,1-dioxo-2,1$l^{6}-benzoxathiol-3-yl]phenol
C1=CC=C2C(=C1)C(OS2(=O)=O)(C3=CC(=C(C(=C3)Br)O)Br)C4=CC(=C(C(=C4)Br)O)Br

It is employed as an electrophoresis tracking dye and also finds its uses as an industrial dye, a laboratory acid-base indicator and a biological stain to detect proteins and nucleic acids (absorbance at 610 nm), particularly when staining living tissues. In gel electrophoresis it can be used as a color marker and as a biological stain it can be used to stain proteins and nucleic acids. Bromophenol blue is an intermediate and is used as an acid-based indicator in the pH range of 3-4.6 where the color changes from yellow to blue.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
It is employed as an electrophoresis tracking dye and also finds its uses as an industrial dye, a laboratory acid-base indicator and a biological stain to detect proteins and nucleic acids (absorbance at 610 nm), particularly when staining living tissues. In gel electrophoresis it can be used as a color marker and as a biological stain it can be used to stain proteins and nucleic acids. Bromophenol blue is an intermediate and is used as an acid-based indicator in the pH range of 3-4.6 where the color changes from yellow to blue.

Solubility
Freely soluble in NaOH. More soluble in methyl and ethyl alcohols ,benzene, and in acetic acid. Slightly soluble in water (0.4g/100g) at 20°C. Soluble in methanol (10 mg/mL.).

Notes
Transition interval: pH 3.0 (yellow) to pH 4.6 (blue)
TRUSTED_SUSTAINABILITY
Melting Point ∼270°C (decomposition)
Beilstein 61698
Merck Index 14,1444
Solubility Information Freely soluble in NaOH. More soluble in methyl and ethyl alcohols ,benzene,and in acetic acid. Slightly soluble in water (0.4g/100g) at 20°C. Soluble in methanol (10mg/mL.).
Formula Weight 669.98
Odor Odorless
Quantity 10 g
Chemical Name or Material Bromophenol Blue
Hazard Category H500
Precautionary Statement P210-P241
EINECSNumber 204-086-2
RTECSNumber SJ7453000
TSCA Yes
Recommended Storage Ambient temperatures

RUO – Research Use Only

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