Invitrogen™ PureLink™ RNA Mini Kit

The PureLink RNA Mini Kit is a column-based kit used to isolate high-quality total RNA from a wide variety of sample types in 20 minutes using standard laboratory equipment.

Supplier:  Invitrogen™ 12183018A

Catalog No. 12-183-018A

Description

The PureLink RNA Mini Kit is a column-based kit used to isolate high-quality total RNA from a wide variety of sample types in 20 minutes using standard laboratory equipment. The kit includes RNase-free lysis and wash solutions that protect RNA from RNases while liberating the RNA from DNA, proteins, and other cellular debris. The fast spin-column workflow is ideal for processing low to mid-throughput batch sizes. The advanced PureLink RNA Mini spin column design allows for maximum sample input (200 mg of tissue) and RNA recovery (up to 1000 μg). This means that users can process both small and large sample sizes with the same RNA isolation kit.

• Process sample inputs from 5–200 mg of animal/plant tissue or from 0.5 x 10⁶ to 10⁸ tissue culture cells with one spin column in 20 minutes
• Isolate more RNA than competitor mini spin columns: up to 1000 μg of total RNA from one column
• Use isolated RNA in most applications without the need for further DNase treatment (very low minimal residual DNA carry over)
• Flexible–optional on-column or post-isolation DNase treatment is available with PureLink DNase Set (Cat. No. 12185010) for easy removal of residual DNA

Extra-large binding capacity enables rapid RNA purification using standard laboratory equipment

The PureLink RNA Mini Kit provides rapid purification of total RNA from a wide range of cells and tissue types to yield up to 1000 μg of purified RNA from a single extraction (see Figure 1). High-quality total RNA can be obtained from mini- to midi-prep amounts of starting material with just trace amounts of residual genomic DNA contamination. The extra-large binding capacity enables one kit to handle most RNA isolation needs in a quick 20 minute protocol without the need for special sample processing instruments.

Easy, optional, on-column DNase treatment for sensitive applications

The PureLink RNA Mini Kit columns are highly efficient for isolating high quality total RNA while removing the majority of genomic DNA. In general, most applications requiring RNA from animal tissue or mammalian cell lines do not require additional DNase treatment. However, some applications such as gene expression analysis by qRT-PCR without intron-spanning primers or working with samples from organisms with very small or no introns may require more complete removal of residual contaminating DNA. The PureLink DNase Set (12185010) allows for convenient on-column digestion of DNA during the RNA isolation protocol. Treating with DNase while 'on-column' is easier and allows higher RNA recovery than treating with DNase after the RNA has been isolated. The PureLink DNase Set can also be used to remove residual DNA from RNA that has been previously purified. Both the 'on-column' and post-RNA purification workflows are options available with the PureLink DNase Set.

Simplified, non-toxic RNA purification

The PureLink RNA Mini Kit utilizes non-toxic guanidine-isothiocyanate lysis buffer to protect the RNA during the isolation steps. Special RNase-free reagents combined with certified RNase-free silica membranes in a unique column configuration allow for a safe and easy procedure that can typically be completed in less than 20 minutes without the need of hazardous phenol/chloroform extraction, CsCl centrifugation, or LiCl or alcohol precipitation.

The PureLink RNA Mini Kit is recommended for use with the Homogenizer (Cat. No. 12183026), designed to homogenize cell or tissue lysates via centrifugation, prior to nucleic acid purification. The Homogenizer is especially effective for clarifying particulates from plant tissues.

Specifications

• Content And Storage: • 125 mL Lysis Buffer; store at room temperature
  • 50 mL Wash Buffer I; room temperature
  • 15 mL Wash Buffer II; room temperature
  • 15.5 mL RNase-free Water; room temperature
  • 50 PureLink Spin Cartridges with Collection Tubes; room temperature
  • 50 Collection Tubes; room temperature
  • 50 Recovery Tubes; room temperature
• Purification Time: 20 min. (After sample homogenization)
• Sample Type: Bacteria, Blood, Cells, Plant, Tissue, Yeast, Enzymatic Reactions
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Product Type: RNA Mini Kit
• Scale: Mini
• Starting Material Amount: Bacteria: ≤10⁹ cells, Blood: ≤0.2 mL, Cells: ≤5 x 10⁷, Plant: ≤250 mg, Tissue: ≤200 mg, Yeast: ≤5 x 10⁸ cells, Enzymatic reactions: ≤1.2 mL

• Isolation Technology: Silica Spin Column
• Elution Volume: 30–300 μL
• Final Product Type: Total RNA
• For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
• Quantity: 50 Preps
• Shipping Condition: Room Temperature
• Yield: Up to 350 μg

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.