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Abnova™ SREBF2 Transcription Factor Assay Kit
SREBF2 Transcription Factor Assay Kit is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates.
Supplier: Abnova™ KA1379
Description
- A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA)
- A specific double stranded DNA (dsDNA) sequence containing the SREBP response element is immobilized onto the wells of a 96-well plate
- SREBP contained in a nuclear extract, binds specifically to the SREBP response element
- SREBP is detected by addition of a specific primary antibody directed against SREBP
- A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm
- This product is for laboratory research use only: not for administration to humans
- This kit will perform as specified if stored as directed and used before the expiration date indicated on the outside of the box
Specifications
SREBF2 Transcription Factor Assay Kit is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. | |
SREBF2 Transcription Factor Assay Kit | |
Functional Study | |
SREBF2 | |
1 Kit |
ELISA (Standard) | |
15 Reactions | |
6721 | |
Transcription factor antibody binding buffer (10X), Transcription factor binding assay buffer (4X), Transcription factor reagent A, Transcription factor SREBP-2 positive control, Transcription factor SREBP-2 primary antibody, Wash buffer concentrate | |
Store the kit at -20°C temporarily. For best results, store the components as described in the protocol. |
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