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  8. 3,3',5,5'-Tetramethylbenzidine, 98%, Thermo Scientific Chemicals

3,3',5,5'-Tetramethylbenzidine, 98%, Thermo Scientific Chemicals

Catalog Number AAA1386803
Click to view available options
Quantity:
1 g
5 g
25 g
54827-17-7
C16H20N2
240.35
MFCD00007748
UAIUNKRWKOVEES-UHFFFAOYSA-N
3,3',5,5'-tetramethylbenzidine, 3,3',5,5'-tetramethyl-1,1'-biphenyl-4,4'-diamine, 3,5,3',5'-tetramethylbenzidine, tmb, 3,3',5,5'-tetramethyl benzidine, tmb substrate, bm blue, ccris 4727, tmb plus, tmb, insoluble
41206
4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline
CC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C
54827-17-7
C16H20N2
240.35
MFCD00007748
UAIUNKRWKOVEES-UHFFFAOYSA-N
3,3',5,5'-tetramethylbenzidine, 3,3',5,5'-tetramethyl-1,1'-biphenyl-4,4'-diamine, 3,5,3',5'-tetramethylbenzidine, tmb, 3,3',5,5'-tetramethyl benzidine, tmb substrate, bm blue, ccris 4727, tmb plus, tmb, insoluble
41206
4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline
CC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C

Description

3,3',5,5'-Tetramethylbenzidine, 98%, or TMB, CAS # 54827-17-7, is a colorimetric reagent used in laboratory-based substrate detection.

Excellent colorimetric substrate for detection of horseradish peroxidase labelled probes; particularly useful in ELISA techniques, blue result can be read spectrophotometrically at 370 or 650 nm3,3',5,5'-Tetramethylbenzidine is used as a clinical reagent for testing blood. It is also a chromogenic substrate which is used in staining procedures in immunohistochemistry. Further, it is used as a visualizing reagent in enzyme-linked immunosorbent assays. In addition to this, it is used as a replacement for carcinogenic compounds such as benzidine and o-phenylenediamine.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description
• 3,3',5,5'-Tetramethylbenzidine, also known as 4,4'-Diamino-3,3',5,5'-tetramethylbiphenyl or TMB, is a colorimetric substrate for detection of horseradish peroxidase-labeled probes
• Oxidation of 3,3',5,5'-Tetramethylbenzidine results in a pale blue end product that can be detected at 370 or 655 nm. Sulfuric acid (H2SO4) is used to stop the oxidation reaction and stabilize the reading, resulting in a yellow color that can be read at 450 nm
Application
• 3,3',5,5'-Tetramethylbenzidine is used as a chromogenic visualization agent in enzyme-linked immunosorbent assays (ELISA) or immunohistochemistry (IHC) techniques
• 3,3',5,5'-Tetramethylbenzidine has been used to detect molecules in aqueous samples such as copper ion and hypochlorite
TRUSTED_SUSTAINABILITY
Melting Point 168°C to 171°C
Quantity 1 g
Beilstein 2808541
Sensitivity Light and moisture sensitive
Solubility Information Soluble in ethyl acetate,ethanol,acetone,chloroform,toluene and dimethyl sulfoxide. Insoluble in water.
Formula Weight 240.35
Percent Purity 98%
Chemical Name or Material 3,3',5,5'-Tetramethylbenzidine

Safety and Handling

Hazard Category H315-H319-H332-H335-H341
Hazard Statement GHS H Statement
H302-H315-H319-H335
Harmful if swallowed.
Causes skin irritation.
Causes serious eye irritation.
May cause respiratory irritation.
Precautionary Statement P201-P202-P261-P264-P264b-P270-P271-P280-P301+P312-P302+P352-P304+P340-P305+P351+P338-P308+P313-P312-P330-P332+P313-P362-P501c
EINECSNumber 259-364-6
RTECSNumber DV2300000
TSCA Yes
Recommended Storage Ambient temperatures; Store under Nitrogen
SDS

RUO – Research Use Only

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